Wednesday, August 26, 2020

Brave mother Essay Example | Topics and Well Written Essays - 1250 words

Valiant mother - Essay Example of the cutting edge world, the job of the mother in the lives of her youngsters has come to reduce, particularly with the strengthening of ladies and their taking up of jobs, which were recently saved for men. The cutting edge world has achieved a lot of difficulties to parenthood, and a portion of these provokes will in general rotate around the way that moms are either working fulltime, or that they are attending a university to encourage their instruction. While in past ages the odds of couples separating were incredibly thin, in the contemporary one, it has gotten ordinary, with numerous relationships self-destructing in brief timeframes. At the point when this occurs, it has become the standard for moms to be given full authority of their youngsters, which has prompted the weight of the bringing up of kids to turn out to be exclusively the area of the mother. This circumstance has become amazingly troublesome particularly when one thinks about that numerous moms need to adjust e ither work or school with the bringing up of their kids; something which is getting very hard to accomplish particularly for those families that need a pay. Today, moms are confronted with the undertaking of bringing up their youngsters, yet in addition their satisfying their commitments to their managers so as to gain a salary. This raises the requirement for moms to adjust their work and family life. So as to accomplish this, numerous moms have searched for options, which won't just empower them to work adequately, yet in addition have the option to invest energy with their youngsters. One of the manners in which that numerous moms appear to embrace is that of telecommuting. Numerous moms have decided to work from home and this has guaranteed that they can invest more energy at home working during when they would have been driving to work. This has empowered them to dispose of work quicker so when their kids return home from school, they discover their moms allowed to invest significant energy with them. Working moms have likewise taken to utilizing one schedule for

Saturday, August 22, 2020

Cakewalk

The nineteenth century was brought by an extraordinary rise of music that would change history. In the late 1980’s an adjustment in cadence developed in playing piano music. Jazz piano was presented as another well known approach to play the piano quick and shallow. The measures were utilized to sixteen beats simply like European partners in spite of the fact that the music was supposed to be the â€Å"afro-American† variant of the polka. In this idea the generalization is drawn as â€Å"ragtime† being related with African American people.Based on the beats in the music which created a sousa style walk, jazz turned into the song used to play out the cakewalk move. In this article the depiction of the cake walk is â€Å"an Afro-American move at first dependent on an exquisite, adapted spoof of southern white cultured manners†, the move is generalized as being made for one gathering of individuals, and that being African Americans. The principal African Amer ican arranger to create jazz music was Tom Turpin which likewise brought along vocalists to turn into the voice of this melodic type of ragtime.Following the vibe of this music, the cakewalk was constantly performed which at that point opened entryways for different kinds of moves and tunes later known as jazz. The article â€Å"Cake Walk, Shimmy, and the Charleston† the portrayals accentuation how moves performed by African American ladies brought solidarity among white and dark crowds. Artists were alluded to as â€Å"Babylon girls† who communicated a positive manner by which African American ladies were being perceived for their sex and race. They communicated in these melodic structures to show dark opportunity and feel a piece of mainstream music.The generalization made in this article depended on the sexuality introduced in the cakewalk move by African American ladies. Many portrayed them as to be â€Å"sexually straight to the point and obstinate, to be figures of freedom. † in all actuality the African American ladies were only an age expelled from servitude in which they needed to manage generalizations from both from white individuals about their sexuality and dark individuals about dark restraint for decency. Moving just permitted them to commend their opportunity and communicate freely.This period of moving and music in the late 1800’s and mid 1900’s carried European styled music blended in with African American mood to starting the jazz time to come. The image on the sheet music doesn’t show any negative analysis. In the symbolism ladies and men are dressed pleasantly with tall top caps and long dresses. Maybe the ladies are dressed more traditionalist than typical ladies that dress in shorter dresses. The men show wide and enormous lips likely demonstrating an African American race.

Wednesday, August 19, 2020

Best Interview Answers for Sell Me this Pen

Best Interview Answers for Sell Me this Pen There are three things which fill people with horror: death, public speaking, and job interviews. Everything about a job interview makes people very anxious. Knowing that the interviewers are watching your every move and scrutinizing your resume makes you feel like a bug under a magnifying glass. It can be so uncomfortable. Now imagine yourself seated before your interviewers, a cold sweat running down your back due to all the anxiety, and then one of the interviewers takes out a pen and says, “Sell me this pen.”This is a very common question in sales interviews, yet it almost always catches the interviewee flat footed. Your mind goes blank, your stomach fills with ice and your throat constricts. A sweat starts forming on your forehead. What do you say? How do you handle this new development? Fortunately, this question doesn’t have to be so disconcerting. Today, we take a look at some of the best answers to this common interview question. WHY DO THEY ASK THIS QUESTION?Like I me ntioned, this question is often asked to applicants interviewing for jobs in sales and marketing. There is one main reason behind this question. Your interviewer wants to know whether you can sell. The question puts you under pressure, because the implication here is you either convincingly sell the pen or lose the job opportunity. One of the key qualities a good salesman should have is grace under pressure, which is why you want to ace this question. Answering the question correctly will demonstrate your sales skills, your communication skills, your mindset, your level of enthusiasm, and your ability to get into your customer’s head. THINGS TO REMEMBER WHEN ANSWERING THIS QUESTIONBelow are some things you should keep in mind when answering this question:1. Show Your EnthusiasmIt’s understandable if you momentarily get knocked off your game when a question like that comes at you unexpectedly. However, you should keep in mind that any good salesman is always passionate and enthus iastic about their product. Therefore, even if your first instinct will be panic, control your panic and instead try to bring out your positivity. In doing so, you transfer your positivity to the product, making it attractive. It also communicates your confidence and encourages the customer to trust you. A good way to show positivity is to start by smiling. Talk highly of your product, but don’t oversell it. Watch your body language. Nod, laugh, maintain eye contact â€" anything that communicates your enthusiasm and shows the customer that he or she has your attention.2. Customize Your PitchKeep in mind that you are not selling the pen to an unknown person. Your buyer (the interviewer) is right there in the room with you. Don’t use any crammed formula â€" customize your pitch for the specific audience. Ask her questions. Cultivate a rapport. Understand what she wants or needs, then use it to sell her the pen.Use what the interviewer tells you to convince her that she needs this pen you are selling. Remember, getting her to buy the pen remains your top priority. Empathize with whatever she says and use that to sell her your pen. By doing this, you show that you are aware that the customer is the most important aspect of any sale. The best salesman focuses on the customer’s needs and ignores his own ego. What the customer wants is always at the top of his mind, and he uses that information to make his sale.3. Always Bounce BackSince this is a job interview and not a real buyer-seller situation, sometimes the interviewer will treat your pitch with hostility or refuse to play along. When you try to ask him questions, he might brush you off. When you try to cultivate a rapport, he might tell you to get to the point or get lost. Don’t let that throw you off your game because that’s exactly what he wants. Don’t get frustrated. Instead, you should remain focused on your goal â€" selling the pen. Find a way to circle back to your pitch. Just take care not t o come off as pestering the customer. Be genuinely affable and your tenacity will come off as good-natured and even inspiring.4. Emotion is KeyThere are two reasons why people buy things:Because they need them.Because buying these things gives them a feeling they crave.The first option is for necessities. The second one is for everything else. If you have a car in your garage, you don’t need a Ferrari. You don’t need china plates when regular plates are available. Why do you keep buying things you don’t need?   The answer to that question is the key to the entire advertising industry.The interviewer probably owns several pens. This particular one is not a necessity. If you want them to buy your pen, find a way to get them emotionally attached to it. Find a way to make them feel that buying it will give them pleasure. They key to infusing your sale with emotion is to use stories. Stories are reservoirs of emotion. A good story can make people weep, laugh, or feel pride. By usin g a story to create emotional value for the pen, you might even get the chance to sell it at a higher price than it’s worth. This is the definition of marketing.REAL LIFE EXAMPLES OF THE BEST WAY TO ASNWER THIS QUESTIONNow that you know why interviewers ask this question and what you should include in your answer, let us take a look at how to actually answer the “Sell me this pen” interview questions. Below are some real life examples of answers to this question by applicants who went on to get hired.  EXAMPLE 1Interviewer: [taking a pen from his pocket and handing it to you] now, sell me this pen.[You take the pen, put it in your pocket. Take a piece of paper, hand it to the interviewer.]You: Hello Chris, I’m a big fan of yours. I really loved your article on the effects of technology in the advertising industry. Can I get your autograph?Interviewer: Sure [starts searching in their pocket for a pen]. Seems like I didn’t carry my pen.You: [taking out the pen] Oh, I have a pen right here, but it will cost you a dollar.Interviewer: Great, I’ll take it.What you have done in this example is to create a need. It doesn’t have to be an autograph, you can think of anything that requires the interviewer to use a pen. By creating a need, you make the interviewer realize that she needs your product for a practical reason. However, things might not always go as expected. The next example spices things up a little bit and shows how you can bounce back if things don’t go as you anticipated. EXAMPLE 2Interviewer: [taking a pen from his pocket and handing it to you] now, sell me this pen.[You take the pen, put it in your pocket. Take a piece of paper, hand it to the interviewer.]You: Hello Chris, I’m a big fan of yours. I really loved your article on the effects of technology in the advertising industry. Can I get your autograph?Interviewer [taking out another pen]: Sure, I have a pen right here.You: How long have you had that pen?Interviewer: A month.You: L ook at it. It looks a little beat up. Don’t you think that a new month requires a new pen?Interviewer: It’s just a pen.You: Really? Just a pen? Think about it. All the things you do with your pen. All the important deals you sign. All the useful notes you jot down. It’s not just a pen. It’s a part of your life. It’s a key part of your success story.Interviewer: What are you saying?You: A new pen means a new mindset. It’s like a fresh start. With a new pen, you can attack the new month with vigor. Each new deal you sign will feel special. Every piece of information you jot down will feel like the best thing you have ever written down.Interviewer: Interesting. I can’t go shopping for a new pen right now. I will get one on my way home this evening.You: No worries. I have one with me right here. [You take out your pen]Interviewer: Wow, looks like a lovely pen, how much is it?You: Just a dollar.Interviewer: Okay, I’ll take it.Just because things don’t go according to pl an doesn’t mean you should give up. Instead, you should find a way to take the conversation where you want it to go. Note how this example makes the interviewer aware that she needs a new pen by calling into question the value of her old one. However, don’t say anything discourteous about what the client currently owns. Be very polite in opening the customer’s eyes to see why he or she needs an upgrade. Note how the example makes the sale by simply showing the emotional benefits the interviewer stands to gain from buying your pen. This is marketing â€" telling stories about your products.EXAMPLE 3Interviewer: [taking a pen from his pocket and handing it to you] now, sell me this pen.You: Hi, my name is [your name]. Interviewer: Hello [your name].You: This is a nice office you have here. You must be a very valuable member of this company.Interviewer: Thank you. Can I help you with something?You: I am from a pen manufacturing company and we are currently doing a poll. We want to find out what people hate or love about their pens. Not ours necessarily. A general poll. Any pens. Let’s start with what you hate about some of the pens you have used.Interviewer: Well, some of them write too faintly. Some of them have tips that are too sharp and they tear up the page you are writing on. Some of them don’t last. Some of them have a tendency to leak.You: And what do you love about the pens you do enjoy writing with.Interviewer: They write cleanly and clearly. Their tips are soft against the paper. They are comfortable to hold. And they are durable.You: What if I told you that I have a pen with me that is exactly like your description of your ideal pen?Interviewer: I say I want to see for myself. You [taking out your pen]: Here it is.Interviewer [Taking the pen]: Can I test it?You: Be my guest.Interviewer: You are right. It’s a good pen.You: Only two dollars, but for you I will make an exception and make it a dollar.Interviewer: Sounds like a deal.The first th ing you have done in this scenario is introduce yourself. Being polite is critical to making a sale. It indicates that you are a positive human being who is at home in any situation. Politeness communicates self-mastery as well as good manners. It gets you in the door. The next thing you have done is give the interviewer a compliment. For a compliment to work its magic, it has to be genuine. Don’t say something generic. Pay attention to your surroundings and to your customer and find something about him you admire. Genuine compliments make people feel warm, pleasantly surprised, and genuinely appreciated for who they are. This is how you break their defenses.The next thing you have done is tell the customer why you are there, and asked him a question that makes him introspect about the product. In doing this, you have made him think about what he likes and doesn’t like in pens. This allows you to present your pen as the solution to a problem he didn’t realize he had until you showed up. The interviewer had probably never reflected on what he hates about terrible pens. Your clever question makes him realize he has a problem: bad pens. The solution: your pen. Note also that what you have delivered here is a pitch custom-made for this customer, and that’s what makes it a great sales technique.EXAMPLE 4Interviewer: You say you are a great salesman, sell me this pen.You: Hi, my name is [your name]. Interviewer [somewhat rudely]: What do you want?You: I work at a pen manufacturing company and â€"Interviewer: We don’t need any pens here.You: Can I compliment you on the beauty of your office?Interviewer [grudgingly]: Thank you. Get to the point, will you?You: Well, we are doing a survey. We want to find out what people love or hate about their pens.Interviewer: What’s the big deal? They are just pens!You: That may well be so, sir. But at [name of your fake pen company], we take pens very seriously. Pens are our whole life. When we sleep at night, we dream of pens. During the day, we are constantly thinking about how to make better pens. When we go about in public, we are observing our competitors’ pens and trying to think of how to make better pens than them.Interviewer: So you love pens, huh?You: If we can make better pens, we believe we can make a small dent in the universe. It might be small but it’s there nonetheless. When pens work perfectly, you are filled with a sense of well-being right?Interviewer: I don’t know if I would call it that.You: When your pen breaks easily because it’s made of brittle material, or scratches the page you are writing on, or leaks, or writes too faintly, how does that make you feel?Interviewer: It makes me very angry.You: So it destroys your equilibrium?Interviewer: You could say that.You: Which means that a good pen   puts you in a good mood?Interviewer: I suppose.You: How would you love to use a good pen to write and sign your important documents?Interviewer: That would be great.You: Here i s a great pen [taking out the pen]. Just one dollar. You can go ahead and test it.Interviewer: Well done.The main lesson from this example is persistence. Polite, respectful persistence. Note how you melt the interviewer’s hostility with a simple compliment. The conversation goes from “I don’t want to listen to you” to “hurry up and tell me what you are here to say”. This is what it means to always maintain your enthusiasm. After the interviewer expresses his contempt of your attempt to cultivate a meaningful debate about pens, you don’t quit. Instead of giving up, you pivot from your intention of asking him about his likes and dislikes to expressing your own passion for pens. This is a technique you use when the customer is uncooperative. When the customer does not want to show interest in your product and even refuses to answer your questions, it is up to you to supply the conversation. Just ensure whatever you say circles back to your main objective: making the sale You should also take note of how this example uses logic to break down the interviewer’s walls. Step by step, you demonstrate the importance of good pens, making him realize that he needs a good pen, that it will add value to his life. That’s how you convert an uncooperative prospect into a satisfied customer â€" through persistence, tact, positivity, and a quick-thinking mind.EXAMPLE 5Interviewer: If you want the job, sell me this pen.You: Hi, my name is [your name]. Interviewer: Hi [Your name]. That’s a nice name. Where are you from?You: I grew up in [your home]. And do you know what I miss the most about my childhood?Interviewer: No. What?You: Writing in my diary. I had this gorgeous pen. I loved it. I haven’t been able to find one quite like it â€" until very recently.Interviewer: Why? What happened recently? Did you find that pen?You: No. Better. I actually found a pen I like better. A pen I can create new golden memories with. Interviewer: Are you trying to sell me a p en? Are you a salesman?You: Yes. But this is more than a pitch. I have sold many products in my life. But now and then, a product comes along that alters your worldview. It blows every single product you used before it out of the water.Interviewer: I have to do some work. Do you mind? [Turning on her laptop, ignoring you]You: I am quite sorry for disturbing you. But before I leave, can I show you something?Interviewer: Okay.You [taking out the pen and handing it to her]: I liked your name. Write it on this piece of paper.Interviewer [writing]: The pen writes very nicely.You: Like I told you. It’s a great pen. And it only costs one dollar.Interviewer: Well done.This script is another good example of persistence and the ability to bounce back. When the interviewer asks you about where you come from, you find a way of bringing the conversation back to your goal. You are here to sell the pen and that’s all that matters. Next you captivate the interviewer with a story that captures h er on an emotional level. She is intrigued by the story of your childhood pen. It makes her think of her own childhood.When she shows disgust for your sales tactics after she figures out all you want to do is sell her a pen, you find a way to make your quest look noble. You do not deny that you are a sales professional, but embrace it instead. As you do so, you express why this particular product is so special. Next she tries to ignore you, and that’s when you decide to go for the kill since she has become uncooperative. Respectfully, you ask if you can show her something. The sense of mystery intrigues her. Next you compliment her name and ask her to write it down for you, accomplishing two things at the same time: you get to make her feel good about herself and also to test your product.By finding a way to always bounce back in spite of the customer’s attempts to ignore you, discourage you, or make you digress, you end up making the sale. Being a good salesperson is about keep ing your eye on the prize and using any means at your disposal to make the sale. Use what the client gives you. And if the client refuses to give you anything, use the surroundings. If the surroundings are not useful, come up with something off the fly. Anything to make the sale.KEY ACTION STEPSThe next time you find yourself having to answer this question in an interview, you can easily answer it by following the steps below.Ask questions to gain information: Asking questions helps you to learn more about the customer (interviewer), therefore allowing you to customize your pitch to the customer. Asking questions also helps you uncover any needs or objections the customer might have.Create a need: The next step is to come up something to make the customer realize that they need the pen, or any other product you might be asked to sell. You don’t have to explicitly ask the interviewer to do something that requires them to use the pen at that moment. The aim here is to make them real ize why they need a pen.Appeal to emotion: Find a way to create an emotional connection to the pen or whatever you are told to sell. Instead of talking about the features of the pen, talk about the benefits of these features, as well as how using the pen will make the interviewer feel.Get the interviewer thinking positively about your product: Find a way to get the interviewer to share what they like and dislike about products in the same category (for instance, pens in general) and then tie the features they like to what you are selling. Circle back to your main objective: Even if the interviewer finds ways to deflect your sales attempt, find ways to circle back to your main objective of selling him the pen. CONCLUSIONBeing a salesman is a bit like being a samurai. Like a samurai, you are loyal to a master: your company. Your mission (selling your company’s product) is your obsession. You should be ready to do whatever it takes to make your sale. This is what the interviewer is t rying to find out when they ask you this question. Do you have what it takes to sell? The examples shown above give you real life word by word scripts you can use to answer this question, as well as insights into what makes them good answers to this question. Therefore, next time an interviewer asks you this question, instead of getting caught unawares, you should have some great ideas on how to wow the interviewer with your sales skills.

Sunday, May 24, 2020

The Best Sandwich Ever - Free Essay Example

Sample details Pages: 3 Words: 1014 Downloads: 2 Date added: 2017/09/20 Category Advertising Essay Type Argumentative essay Did you like this example? The Best Sandwich Ever The first sandwich was made in 1st Century B. C. by the famous rabbi, Hillel the Elder, during Passover. The sandwich was a remembrance of the Jews’ suffering before their deliverance from Egypt. This wonderful sandwich consisted of a mixture of chopped nuts, apples, spices and wine on two matzos, and was to be eaten with bitter herbs. Although the rabbi’s sandwich may not seem appetizing to the tastes buds of todays greater community we are grateful for his idea because now we have the creations every little kid holds in his/her lunch box today. The sandwich world has evolved into sandwiches as simple as the favored bologna sandwich to ones as complicated as the sandwich I like to call the SASS or the Super Awesome Supreme Sandwich. Sandwiches are filled with millions of flavors, and have different textures that make it the most popular item in a lunch boxes all over the world. The most important part of the sandwich dining experience is the fillers of the sandwich, starting with the meat. In the Super Awesome Supreme Sandwich there is one main meat that controls the rest of flavors, this meat is called salami. Not just any type of salami though, the salami in this sandwich has to be spicy and colorful. This salami is filled with peppers of all different types. Pepper is something that you have to be veracious with, too much and it’ll over power the sandwich, not enough then the meat will just be taking up space. In addition to the pepper the salami has to have a slightly pungent taste of garlic and salt. Garlic and salt will balance the flavor of the pepper and allow for one to explore new taste buds. The garlic and salt allow for a tangy contrast to the pepper making just these few layers of meat make your mouth water. In addition the taste of the salami the look of it is very important. Each seasoning has a different look and allows the meat to become a rainbow or color and flavor. The meat itself should be a red-orange color with black and white spots. These spots are where each entire piece of flavor is kept. In addition to meat there has to be veggies in this sandwich to add color and crunch. The Super Supreme has two very important veggies, shredded lettuce and ripe red tomatoes. Lettuce in this sandwich has to be freshly washed with still a little bit of water on it to contain the pristine taste. This lettuce is of a mellow, hardy, green texture. The heirloom tomatoes are vibrantly red with juicy seeds and firm skin. The color combination of the Italian salami, the hardy lettuce and the red heirloom tomatoes bring an aesthetic and redolence to this experience. The next important part of this sandwich is the condiments that add a zing of flavor and serve as an accoutrement to the palate of the diner. Many would slather on a mayonnaise but here an aioli is lightly applied to the bottom exterior casing of this sandwich. Typically an aioli is a mixture of mayonnaise a nd garlic but accompanied with French’s Mustard the combination provides color, moisture and flavor. Too completely accessories the Super Supreme there must be a melody of complimenting herbs and fruits. Olives, yellow bell pepper, rosemary, thyme, basil, marjoram, and oregano each finely chopped and drizzled throughout the sandwich allow for an intellectual taste of Italy. The Super Awesome Supreme Sandwich casing gives off the first impression and holds together a tantalizing dining experience. A sourdough loaf is chosen as it will provide a sturdy and stable coat for the Super Supreme. When selecting a loaf one must use all of the senses but one to test the freshness of the bread; the sense of touch to check firmness of the bread, the sense of smell to acquire the aroma of a virgin loaf, the sense of sight to view any imperfections and the sense of hearing to survey for a robust crunch. Once one is tempted to use the sense of taste, the correct loaf has been selected. N ote that this loaf is cut into slices offering an open face three by five canvas that has been toasted to perfection. A perfectly toasted loaf is found when the bread is browned with slightly darker columns seen, or grill marks. The crust that has now become crunchy and darkened will compliment the inner bread’s light crisp and browning. The Super Awesome Supreme Sandwich is a sandwich of many layers and pieces such as that of an outfit of a sophisticated lady. She always has her coat and purse, the bread. Her coat is what keeps her warm and safe, while the purse is where everything is held that she may need throughout the day. The bread holds everything together, keeps it warm, safe and easy to carry. Also a lady looks put together, her outfit is always clean and color coordinated, the meat. The outfit is always appropriate for the occasion never over or underdressed and ready for anything. The meat put inside a sandwich should never over power the rest of the ingredie nts inside but still be the main aspect of the meal. In addition she always has her accessories, the condiments. The accessories are where her true colors are shown and the entire outfit is matched together. Condiments are like accessories because they complete a sandwich, without them it would just be incomplete. The smear that is applied is like the shoes, something absolutely necessary and if carefully chosen can define a sandwich. Fruits and veggies are like the earrings, something a lady should never leave home without. The different flavors of the sandwich made by rabbi Hillel the Elder has evolved just as the attire of a lady. With every century the variety of flavors and textures expands allowing for each generation to venture out and have a creation of their own. The Super Awesome Supreme Sandwich is a prime example. Don’t waste time! Our writers will create an original "The Best Sandwich Ever" essay for you Create order

Wednesday, May 13, 2020

Walter Elias Disney s Life - 1693 Words

Walter Elias Disney was born in December 5, 1901 in Hermosa, Illinois. Walt Disney was born to his two parents Elias Disney, who was an Irish Canadian, and Flora Call Disney, who was a German American. His siblings are Roy O. Disney, Raymond Arnold Disney, Herbert Arthur Disney, and Ruth Flora Disney. For most of his childhood, he grew up in Marceline, Missouri. As a child, Walt would sell his drawings, painting, and pictures to his neighbors and family friends. In 1911, he moved to Kansas City, when he developed a love for trains. His uncle Martin was a train engineer who worked the route between Fort Madison, Iowa and Marceline, Missouri. That summer Walt worked a summer job with a railroad selling snacks and newspapers to all travelers. Walt moved, once again, attending the McKinley High School in Chicago. In his new school, he took photography and drawing classes and was the cartoonist for his school’s paper. During the night, he took classes at the Chicago Art Institute. At 16, Walt dropped out of high school to join the Army, but was rejected for being underage. After being rejected, he joined Red Cross and was sent to France for a year, where he drove an ambulance. In 1919, Walt returned to America, where he moved back to Kansas City to pursue a career as a newspaper artist. Roy Disney, Walt’s brother, was working at Persmen-Rubin Art Studio, where he met cartoonist Ubbe Eert Iwerks. From there, Walt worked at Kansas City Film Ad Company, where he made commercialsShow MoreRelatedWalter Elias Disney s Life1216 Words   |  5 PagesWalter Elias Disney was born on December 5, 1901, in Chicago, Illinois. His father was Elias Disney, an Irish-Canadian, who was a building contractor, and his mother, Flora Call Disney, was German-American, who was a public school teacher. Walt was one of five children, four boys and a girl. 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Walt Disney is a wonderful and patriotic man whoRead MoreEntrepreneurship Is The Finding Of An Idea And Building It Into Action Essay1460 Words   |  6 Pageshigher the profits. Entrepreneurs break away from tradition through their unique offerings of new goods and services and indirectly support freedom by reducing dependence on obsolete systems and technologies. This results in an improved quality of life, greater morale and economic freedom Entrepreneurs, like Bill Gates, have used their money to finance good causes, from education to public health. The qualities that make one an entrepreneur are the same qualities that motivate entrepreneurs to payRead MoreWalt Disney And The American History1044 Words   |  5 Pagesthings, or get something going.† (LeeBron) Disney stated that when he realized that he wanted to make a difference in people’s lives. Disney never wanted to be boring or be known as the man who did not have a childhood and was always pushed around by his father. Disney wanted to be successful in life, he wanted the name ‘Walt Disney’ to be remembered, and it will be forever. Disney has achieved many important and exciting accomplishments within his life. He created â€Å"full length animated films, shortRead MoreWalt Disney: A Key to Childhood Hearts2466 Words   |  10 Pagesliving room, and you hear the famous Disney music coming from the television that your child is placed in front of. You start to wonder where did your favorite Disney movies come from and how were they created? What was Walt Disney’s childhood like when he was younger that made him want to animate and put together cartoons and movies? Where did his career start off from and how did he get to such a place? But the important question, who took over after Walt Disney passed? These are some questions thatRead MoreThe Life And Accomplishments Of Walt Disney1934 Words   |  8 PagesWalter Elias Disney was always seen as a very imaginative and outgoing person at any point in his life. Walt loved life and would make appearances at Disneyland all the time to make sure employees were being kind and courteous to the guests while managing to talk and ride rides with children that looked up to him. Never in his life would Walt give up on something, which made him very stubborn at times, but a good businessman with the guidance of his brother, Roy. All of these traits made Walt DisneyRea d MoreWalt Disney : An American Motion Picture1226 Words   |  5 PagesWalter Elias Disney, also known as Walt Disney, was an American motion-picture and television producer, famous as the creator of cartoon films and Disneyland. His relentless aspiration and determination drove Walt Disney to pour in each ounce of innovativeness into his work. In my opinion, Walt Disney was important because of his creation of Disneyland in order to have an educational as well as amusement value and to entertain adults and their children and his influence on American culture throughRead MoreWalt Disney : A Pioneer Of Cartoon Films And As The Founder Of The Disney Company957 Words   |  4 Pagesimagination ¨ (Disney, 2014). Walter Elias  ¨Walt ¨ Disney is famous as a pioneer of cartoon films and as the founder of The Walt Disney Company. He was an American motion-picture producer, television producer, and a showman. Disney came from humble beginnings but ended up transforming the entertai nment industry. Walt Disney had a challenging childhood, grew up and chose a career that would liven up his life, achieved many accomplishments, and left a legacy for years to come. Childhood Walt Disney was born

Wednesday, May 6, 2020

Elizabeth Bishop’s Poetry Free Essays

Elizabeth Bishop poses interesting questions delivered by means of a unique style. Do you agree? Focus on themes and stylistic features. In my opinion, Elizabeth Bishop has a unique style of asking interesting questions. We will write a custom essay sample on Elizabeth Bishop’s Poetry or any similar topic only for you Order Now Bishop invites us along on the journey with her. She does this by her â€Å"painterly eye† which she has been praised for. In her poems she takes the ordinary and turns it into the extraordinary. As a reader, I wonder why she goes into so much detail. There is a story behind each of her poems. Her poems â€Å"First Death in Nova Scotia† and â€Å"In the Waiting Room† are about childhood experiences. She uses great detail in her poems and we feel like we are apart of it. This can be clearly seen in Bishop’s poem â€Å"The Fish†. â€Å"The Fish† is an example of where Bishop turns something so plain into the extraordinary. She takes fishing and turns it into a seventy-six-line poem. This poem recalls a time when Bishop went fish in a rented boat. Bishop makes a clear statement in the opening line of the poem, â€Å"I caught a tremendous fish†. The adjective tremendous is very effective, I feel. In the first four lines, Bishop stated how she caught a huge fish and stared at it beside her boat. She didn’t haul the fish into her boat. I question why she didn’t bring it straight on board. Bishop’s delight in catching the fish soon gives way to an emotional involvement with the fish. She compares his eyes to her own and she notes that the irises are â€Å"backed and packed with tarnished tinfoil†. The image is emphasized by assonance and alliteration. It was a big personal achievement to catch the huge fish. Bishop began to enjoy her triumph. It was a big moment for her. She imagined that her feeling of victory filled up the rented boat. Meanwhile, the big fish was still partly in the water. Then she did something unusual. She released the fish she had caught: ‘And I let the fish go’. I wonder why she had mercy on the fish and decided to let it go. â€Å"Filling Station† is another clear example of Bishop turning the ordinary into the extraordinary. In this poem Bishop is writing about a family petrol station. The voice in the poem is that of an outsider. The compound words â€Å"oil-soaked† and â€Å"oil-permeated† give us a clear vision of this petrol station. I wonder why Bishop is there in the first place. We become fascinated with the place. In verse two, the speaker sees the family. The image of everything covered in oil is continued. Alliteration is used to describe the sons, â€Å"several quick and saucy and greasy sons assist†, this suggests they have an oily appearance. The speaker begins to wonder if anyone lives here, â€Å"Do they live in the station? †. Bishop looks for and finds evidence of the female touch in verses four and five. We begin to see that there is beauty and love in the most unlikely places. In this male-dominated world, there is care to attention and detail with the mention of â€Å"daisy stitch†. In the final verse the repetition of â€Å"somebody† highlights the importance of the mother. The poem ends with the assurance that everybody is loved and worthy of love. Bishop recalls a childhood experience in her poem â€Å"In the Waiting Room†. This poem is similar to â€Å"First Death in Nova Scotia† as both have a theme of childhood innocence in them†. Perhaps the most immediately striking feature of Bishop’s work is its child narrator describing the seemingly innocuous event of waiting at the dentist’s office while her aunt is in the patient’s room. In this setting, the memory revolves around the narrator reading a  National Geographic  magazine. Bishop writes in uncomplicated, declarative language like â€Å"It was winter. It got dark / early. † that mirrors her age at the time. The poem takes an interesting direction as the child-speaker sees herself as a young woman: â€Å"What took me / completely by surprise / was that it was me: / my voice, in my mouth†. Aunt Consuelo’s cry becomes the speaker’s own cry. The woman and the girl merge into one in a surreal leap of the imagination â€Å"I – we – were falling, falling†. This poem makes us question what it means to be a woman. In â€Å"First Death in Nova Scotia† Bishop presents an extraordinarily vivid memory of a disturbing personal experience. It is winter in Nova Scotia. The dead child has been laid out in a â€Å"cold, cold parlour†. As in â€Å"In the Waiting Room† the voice in this poem is that of a child-speaker. How to cite Elizabeth Bishop’s Poetry, Papers

Tuesday, May 5, 2020

Literature Review Water Consumption

Question: Discuss about theLiterature Reviewfor Water Consumption. Answer: Introduction Background and Overview of Water Consumption In the previous studies, it has been identified that the water consumption in different countries has increased. However, supply of drinking water in the countries is not enough for meeting the demand for the drinking water (Xu et al., 2009). The water shortage occurred in different countries because of the carelessness of the people. Many times, the people misuse the drinking water. The water pollution is the other reason behind the shortage of drinking water. Due to the high water pollution, the quality of the drinking water has decreased, which causes different diseases. Due to the shortage of drinking water, the people in different countries especially in UAE are suffering much. The solutions of this problem are desalination and reuse of grey water. Definition of Water Consumption Water consumption can be defined as the consumption of fresh water, which is taken from the ground or other surface sources of water for temporarily or permanently (Khan, Badr Al-Zubaidy, 2014). The cost of desalination and reuse of grey water is much high, which may affect the economy of the countries. Thesis The increased rate of water consumption is a big problem for the countries and this problem must be solved as soon as possible. Theme 1: The water consumption is playing rule in different aspects In the words of Khan, Badr and Al-Zubaidy (2014), water is the primary source of life. Alkend and Omar (2013) stated that water is the basic need of any life. The function of water can be identified in several areas like, houses, schools, factories and other places. The households use water in order to perform their day-to-day activities like, cooking, washing, gardening, drinking and others. In schools and colleges, water is used for drinking, cleaning and washing purposes. Different factories use water in order to run their production and at the same time to supply the water to the workers for drinking and cleaning purposes. Xu et al., (2009) stated that water is also important for the growth of the plants. Water helps the plants in the photosynthesis process. This means, water helps the plants getting their foods, which is the main source of growth. On the other side, the consumption or use of water has direct effect or relationship with the economies of different countries. In the countries, where water shortage exists, requires desalination or reuse technology to maintain the smooth flow of water supply to the people, plants and animals. Maria Botros has stated in her article Solutions to tackle water scarcity in the UAE that the cost of implementing and processing the desalination and reuse technologies cost much, which can increase the cost of the economy. If the cost in the economy increases, the income level automatically decreases. Theme 2: The Effects of the Water Consumption in Several Aspects The over consumption of water has impacted the life and organisms. Over water consumption created fresh water shortages in different countries, especially in UAE countries. Due to this, the governments in the countries are requiring implementation of advanced technologies, which are costly (Gulfnews.com, 2017). However, the water shortage problem has not been solved completely. In many UAE countries, people are not getting enough drinking water and due to that many diseases are taking place. As per the statistics, every day, 5000 children die due to the shortage of water or due to drinking low quality water. The worlds statistics have shown that in last 50 years, the freshwater consumption has increased by 64000 billion liters. The statistics have also shown that to produce one liter of bio-fuel, near about 4000 litres of water is required (Worldometers.info, 2017). Over use of water creates water shortage, which has direct impact on the climate and environment. The level of global warming has increased due the water shortage. At the same time, the water shortage is also causing threats to the plants. The quality of water is decreasing, which is also affecting the plants as well as people and other animals (Md. Bader, 2011). As the agriculture in the countries depends on water, the water shortage or bad quality of water is also negatively affecting the agriculture sector. Theme 3: The Solutions to Reduce Water Consumption and How Control it In order to solve the water shortage problem, the governments as well as people in the countries require to come forward. The water supply authorizes of the countries can reduce the water consumption by setting a specific consumption standard for each household and factory (Gulfnews.com, 2017). The governments can implement specific water consumption laws in order to control the water consumption. The government can also warn the people in the country to be more careful about the water usage. Along with these, the government can also implement the desalination and reuse technologies in order to solve the problem. These technologies will help to produce more fresh water or drinking water. Conclusion In this study, it has been identified that the level of water consumption has increased much in the present scenario. Due to the over consumption of water, many problems have occurred like, global warming, critical diseases and shortage of drinking water. In order to solve these problems, the governments can set water consumption standards and can implement desalination and reuse technologies. If the water consumption is reduced, then the countries may enjoy several benefits. If the water consumption in some countries is reduced, then the other countries, where the people face water scarcity, can get required amount of drinking water. The people can survive properly. The reduction in the water consumption will also help reducing the global warming and maintaining the standard of natural environment. Reference List: Alkend, R. R., Omar, S. (2013). Total Bacterial Quantification in Homes' Water Tanks.International Journal of Applied Biotechnology and Biochemistry,3(1), 51-74. Gulfnews.com. (2017). Dubai News, Abu Dhabi News, UAE News and International News from GulfNews.com plus Gold rates, sport scores, city guides, prayer times, Dubai financial data, weather forecasts and more. GulfNews. Retrieved 4 February 2017, from https://gulfnews.com/ Khan, S. A., Badr, A., Al-Zubaidy, S. N. (2014, March). Valuation of Chilled Water Condensate through Air Conditioning to Diminish Water Scarcity. InInternational Journal of Engineering Research and Technology(Vol. 3, No. 3 (March-2014)). ESRSA Publications. Md. Bader, F. (2011). The Effects of Different Faucet Aerators on Bacterial Growth and Water Quality, 1(1), 1-92 Worldometers.info. (2017). Worldometers. Retrieved 4 February 2017, from https://www.worldometers.info/ Xu, P., Wang, G., Cath, T., Drewes, J. E., Ruetten, J. (2009). Consider the Pros and Cons of Desalination (PDF).Opflow,35(3), 10-13.

Tuesday, March 31, 2020

Johnsonville Sausage Essay Example

Johnsonville Sausage Essay Johnsonville Sausage – Quality Improvement through Change Situation Post 1980, Ralph Stayer began to observe decline in quality due to inattention of the workers as well as ill-maintenance of the equipment in the production unit. With the help of Mr. Lee Thayer, Stayer has planned to implement a major change in the company’s philosophy. Transition from old philosophy purged Stayer’s responsibilities of decision making by decentralizing the power of decision making. The new philosophy was implemented to bring out changes in various departments of the company viz. management structure, personnel, compensation, manufacturing and also systems and controls. According to Thayer’s new philosophy, performance of the personnel and requirement of guidance to personnel to achieve their objectives were the key elements. Management structure was broken down by hiring experienced personnel for the new superior level management to oversee finance, marketing and operation s functions. Stayer emphasized people development as the core element of the new philosophy. Changes in personnel include development of list of responsibilities by the workers themselves and eliminating the performance review process. These changes in personnel function led changes in compensation with the implementation of â€Å"company performance share† program which increased individual responsibility of workers. However this transition was not in agreement among workers while some are excited about it. In terms of manufacturing changes, lead persons were installed at various departments, eliminating supervisors, to seek information and provide necessary measures to train and improve capacities of workers under designated group. Responsibilities of lead person also include development of budget planning and production requirement planning. Job switching and alternating works were practiced to enrich workers’ capabilities and enhance performance and also job satisfaction. System and controls changes included individuals to develop and be accountable for their own budgets and forecasts and also allow them to analyze performance data. In addition, subordinates were needed to self-evaluate their performance to respective managers. Problem The main issue of the case is the resistance shown by the personnel towards the company’s philosophy transition. According to Stayer, upon implementation of new philosophy, workers at all level were expected to embrace the change. We will write a custom essay sample on Johnsonville Sausage specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on Johnsonville Sausage specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on Johnsonville Sausage specifically for you FOR ONLY $16.38 $13.9/page Hire Writer Instead, the company faced issues related to the final goal of the implementation, unsuccessful organization structural changes, clash of responsibilities among the workers, participation of less number of employees in the company decision making process and finally lack of high job satisfaction. However, these issues were expected to expire once the transition is complete. Palmer Sausage offered its consolidated business to Johnsonville but the issue here was whether Stayer could handle an expanded Palmer contract. Stayer examined the situation by understanding that in order to run the contract the company would require to recruit and train a big set of people along with existing people acquiring new skills and retain high quality on both the in-house and Palmer products. In order to achieve this, Johnsonville needs to run the production six or seven days a week for more than a year until its new plant was ready. On the other hand, the company may need to run the business at risk i. e. , if Palmer canceled the contract with 30-day prior notice leaves Johnsonville not only with massive layoffs and but also huge inventory with no market for it. Analysis As the year 1980 folded, Stayer recognizes to restructure his management structure for the growing company. Stayer also realized that there was decline in the quality of the products and employees were also demotivated to work. Later on, in collaboration with Thayer Lee, Stayer has planned to implement new philosophy which is focused on performance and helps people accomplish their objectives through well-defined job descriptions, clear understanding on performance standards, and providing the required resources. Johnsonville’s new values are based on deep moral commitment to the individual. The main aim of the transition is to augment people capacities, rather than the business. Stayer changed view of his autocratic leadership to a role as a leader delegating his subordinates rather than directing them. Furthermore, the new philosophy also motivated each worker to commit to the company’s betterment. Upon full transition, Stayer expects to create an atmosphere of constant learning and development. The transition also supports creativity, encourages responsibility, and empowers employees to develop solutions rather than simply follow orders. Over the period of time, the company’s organizational structure was transformed in support to the new culture of worker empowerment. Autocratic management was decentralized in order to become more democratic. Conventional management position were replaced by lead persons and their teams who controlled their own compensation, hiring, firing, quality control, and near-and long-term company goals. Several ground-breaking solutions were instigated and evaluated and each member of the company was involved for the company’s success. Moreover, a rewarding compensation system was implemented to retain employees and hone their skills and performance. Over-the-time, Stayer had achieved a complete organizational transformation successfully. In terms of Palmer’s offer, Stayer has two recommendations – either he can accept or decline to take over the Palmer’s business. If Stayer had taken over the business, they might have the advantages of the opportunity for growth and additional revenue. On this regard, private label business offers a 25% return on assets. Contrariwise, the deal clashes with Johnsonville’s recent business strategy which prevents the company to push private label business over 15%. The company could compete for capital with the rest of the business once it crosses 15%. In addition to this, the company, while in contract with Palmer, is producing at huge capacity utilization. If the deal is on then the company might need to execute two long shifts for six or seven days a week in order to process the new business. Recommendation At this stand-off point, Johnsonville could be advised to accept Palmer’s deal which help the former to tap the revenue and growth opportunities. Stayer’s Johnsonville should continue to implement its new philosophy in a way that it does not affect the previous work-culture. During the agreement, Stayer should continue to involve employees in the decision making process to accept the private label business and planning to handle the additional workload as well. Apart from this, Stayer is required to motivate his teams to meet for evaluating staffing requirements, production and quality goals. With the involvement of workers and assigning responsibility for the important decisions, Stayer will help secure their buy-in for tackling the challenges ahead.

Saturday, March 7, 2020

Revisiting Wether, Incidence and Different Than

Revisiting Wether, Incidence and Different Than Revisiting Wether, Incidence and Different Than Revisiting Wether, Incidence and Different Than By Maeve Maddox wether/whether In researching the recent song lyrics post, I came across a comment written by a high school sophomore. (For the information of non-American readers, a high school sophomore is 15 or 16 years of age.) The student said she was writing a research paper on the influence of song lyrics. I certainly hope she looks up the spelling of the conjunction whether before she finishes her assignment; she used it four times in her comment, each time spelling it wether. wether (noun): a castrated ram. whether (conjunction): one use is to introduce an indirect alternative question expressing doubt or choice between alternatives. More at â€Å"Wether, Weather, Whether.† incident/incidence NPR (National Public Radio) announcers are a rich source of nonstandard English. On a recent morning I listened to Sam Sanders report on a pediatrician who prescribes exercise to his overweight patients. One of the doctor’s techniques is to encourage patients to visit local parks. Sanders mentioned that safety is a concern. He said that one of the parks, Kingman Island, â€Å"had 30 incidences of violent crime over the past year.† The erroneous use of incidences for incidents was cleaned up in the transcript, but it can be heard in the audio (3:33). incident (noun): something that occurs. incidence (noun): the range or scope of a thing; the extent of its influence or effects. For example, â€Å"The incidence of poverty among  the aged has consistently been higher than for any other age group in the United States.† More at: †It’s Not the Ox-Bow Incidence† different from/different than/different to A reader asks, â€Å"Is the correct usage ‘different to’ or ‘different from’? Different to seems very common (almost universal), but surely the essence of difference is separation, not convergence, so isn’t ‘different from’ correct?† This question comes up frequently, often with angry attacks on speakers who use the â€Å"wrong† phrase. Of the three, â€Å"different from† is by far the winner on the Ngram Viewer. â€Å"Different to† is heard more frequently in Britain than in the United States. â€Å"Different than† has its American defenders, but the AP Stylebook comes down firmly for â€Å"from, not than.† The Chicago Manual of Style is less dogmatic, but does say, â€Å"The phrasing different from is generally preferable to different than.† More at †Different from, Different to, Different than.† Want to improve your English in five minutes a day? Get a subscription and start receiving our writing tips and exercises daily! Keep learning! Browse the Misused Words category, check our popular posts, or choose a related post below:100 Words for Facial ExpressionsThe Possessive ApostropheRite, Write, Right, Wright

Thursday, February 20, 2020

Strategy, Business Information & Analysis Essay

Strategy, Business Information & Analysis - Essay Example Sri Lanka is one of the few countries in the world, providing free universal healthcare, including both education and healthcare services (Workinsrilanka.lk, n.d.). Development of the healthcare industry is one of the major government priorities and for achieving its goals; the Government has initiated the National Health Master Plan (2006-2016). Government support has contributed significantly to the rapid growth and development of both public and private sectors, improved quality of healthcare services and human capital base in the industry (Workinsrilanka.lk, n.d.). As of 2012, Sri Lanka had 593 government hospitals and 197 private hospitals. The number of qualified doctors in total exceeded 17, 129 (approximately 1,187 persons per doctor) and the number of qualified nurses was 29, 871 (approximately 683 persons per nurse) (Workinsrilanka.lk, n.d.). In 2012, the total expenditure on health care both private and public was approximately US $89 per head, or 3.2% of GDP (The Economis t Intelligence Unit, 2014). Even though the level of expenditure on health is low, Sri Lanka illustrates significant positive trends: increase of life expectancy to 75 years, and fall of mortality rate among children t 9.6 cases per 1,000 births (The Economist Intelligence Unit, 2014). The quality of healthcare services provided in Sri Lanka is known around the world, as this country has high quality clinicians, adopts the latest innovations in medicine, science, and technology (Medicare, 2015). In order to have better understanding of the healthcare industry dynamic in Sri Lanka there was utilised the five forces model. There is a limited number of suppliers of medical devices, consumables and pharmaceutical items in Sri Lanka. In addition to limited availability of suppliers, Sri Lankan’s healthcare industry faces with a problem of limited availability of medical devices, consumables and pharmaceutical items often causing stock out situations. Limited number of

Tuesday, February 4, 2020

Business & Society ( Ethics & Stakeholder management) Essay

Business & Society ( Ethics & Stakeholder management) - Essay Example A.2: A CEO has to lead by example and John Mackey must be aware of this basic ethical tenet. Else, he would neither have taken the trouble to hide his identity nor chosen the Yahoo! Finance Stock Forum, to air his messages. Yet he persisted with this deception in spite of knowing the implications of his activity. The loss of prestige in the event of his being found out probably did not bother him too much. I would qualify his attitude as being ‘indifferent’ in setting high ethical standards for his company. A.3: A firm’s image is the sum total of its products and social responsibilities. Firms go to great lengths to reinforce their positive images in fields such as corporate governance, social responsibility, environmental compliance etc., in order to attract various stakeholders – employees, suppliers, consumers, investors and regulatory authorities. John Mackey’s activities are definitely harmful for the future of Whole Foods. The specific example of the difficulties faced in its attempts to acquire Wild Oats can be seen A.1: The reality of the situation (or, ‘what is’) is that my boss used official resources for his personal purpose, however insignificant it may be. This was not a one time, inadvertent practice, but indulged periodically with the full knowledge of himself and his subordinates. The normative (or, ‘what ought to be’) behavior demands that personal works are not carried out at the expense of the business. A.2: The boss’s practice is not ethical. He is trying to justify his action by rationalizing. Neither his length of service with the company nor the limit put by himself on the maximum postage at 60 cents, offer any justification for his conduct. If this issue is ignored, one has to answer the logical ends up to which such behavior can be accepted as ethical, in an infinite number of situations. A.3: If his argument of low pay being compensated by his unauthorized use of company resources

Monday, January 27, 2020

Putative S-Adenosyl Methionine Dependent Ironâ€Sulfur

Putative S-Adenosyl Methionine Dependent Iron–Sulfur Identification and Characterization of a Putative S-Adenosyl Methionine dependent Iron–Sulfur containing protein from  Methanococcus Jannaschii Qi Liu Research Proposal Dr. MishtuDey Dr. M. Lei Geng Dr. Leonard R. MacGillivray Dr. Amnon Kohen Dr. Daniel Quinn Introduction Biological methane formation is a microbial process that is catalyzed by microbes called methanogens, which belong to the third kingdom of life, the Archaea. Methane is formed at the final catalytic step by methyl-coenzyme M reductase (MCR), in which coenzyme B (CoBSH, N-7-mercaptoheptanoylthreonine) donates two electrons to reduce methyl-coenzyme M. MCR is a 300kDa enzyme, which is composed of three different subunits in an ÃŽ ±2ÃŽ ²2ÃŽ ³2 arrangement and contains 2 mol of the nickel tetrapyrrole coenzyme F430, which are buried deeply within the protein complex[1]. There are five modified amino acids were found out on the ÃŽ ±-subunit and near the active site of MCR from methanothermobacter marburgensis based on the X-ray crystallographic studies. They are 1-N-methylhistitine (HisÃŽ ±400), 5-(S)-methylarginine (ArgÃŽ ±271), 2-(S)-methylglutamine (GluÃŽ ±400), S-methylcysteine (CysÃŽ ±452), where the side chains are methylated and one thioglycine (GlyÃŽ ±445) forming a thiopeptide bo nd (Figure 1). Since the DNA sequence of the encoding MCR gene shows no unsusal condons or unusual codon usages at the positions in which the five modified amino acids were found, the modifications are introduced after translation [1]. According to vivo labeling experiments with L-(methyl-D3)-methionine, people found that the methyl groups from the modified amino acids are introduced biosynthetically from the methyl group of methionine by specific S-adenosylmethionine (SAM) dependent Figure1. Post Translational Modifications in MCR  enzymes. These methyl translational modifications are catalyzed by protein methylases that specifically recognize the amino acid sequences up and downstream of amino acid to be methylated. The genome of methanogens has many open reading frame predicted to be putative methyltransferases, which also agrees with the proposal that there are four different SAM-dependent protein methylases are involved in the post translation modification. A search of six kn own methanogenic genomes led to the identification of conserved open reading frame around the MCR gene cluster. Some methangenic archaea contain two MCR isoenzymes, designated MCR1 and MCR2. This conserved hypothetical protein is found around MCR1 in Methanococcus jannaschii and Methanobacterium thermobacter. The open reading frame from Methanococcus jannaschii, MJ0841 is annotated as a conserved hypothetical protein, which is found to be related to the radical SAM enzyme superfamily. The signature motif of SAM radical enzymes is three cysteine motif â€Å"CX3CX2C† (Figure 2), multiple sequence alignment of the putative gene from methanogens show the conserved CXGFCXXC, which is known to coordinate to [4Fe-4S] cluster. (Figure 3) Figure 2. Multiple Sequence Alignment of MJ0841 Homologues from Different Methanogens Figure 3. [4Fe–4S] cluster coordinated by three-cysteine motif CxxxCxxC. The fourth iron of the cluster interacts with a bound SAM. Specific Aims It is interesting and important to determine the function of this hypothetical protein. We speculate that this hypothetical protein, MJ0841, could be a possible candidate responsible for the post-translation modification of the methylated amino acids, or, is involved in the formation of the thioglycine in MCR. Research Plan, Results and Discussion Expression and Purification of MJ0841 MJ0841, a 1248bp gene, was cloned into pET28a vector. The resulting plasmid was transformed in E.coli BL21(DE3) cells for gene expression. In order to increase the iron content, MJ0841 was also co-expressed with PDB1282. Overnight cell culture grown at 37oC in Luria-Bertani (LB) medium containing both kanamycin (50ug/ml) and ampicillin (100ug/ml) was inoculated, in a 100-fold dilution, into Terrific Broth (TB) media aerobically at 37oC. FeCl3 was also added to a final concentration of 100uM to be as the iron content for iron-sulfur cluster during the growth when OD600 was around 0.3. Protein expression was then induced at OD600 of 0.6 to 0.7 with addition of Isopropyl ÃŽ ²-D-1-thiogalactopyranoside (IPTG) to final concentration of 0.5mM. After overnight incubation at 37oC around 18hours, the cells were harvested by centrifugation at 5000rpm for 30mins at 4oC, and stored at -80oC. The following procedures were all carried out in oxygen free environment at 20oC. Purification was conducted anaerobically at oxygen level always below 2.0ppm in anaerobic chamber. 23g Cell were moved into anaerobic chamber and resuspended in 120mL lysis buffer (50mM tris-HCl, 300mM NaCl, 5% glycerol pH 7.5), and Phenylmethanesulfonyl fluoride (PMSF) 1mM final concentration, 3 tablets of protease inhibitor, 2-mercaptomethanol 10mM) for 15mins. The cells were lysed by sonication for 15mins followed by centrifugation at 30,000 rpm for 40mins at 4oC to remove the cell debris. The supernatant was applied to a packed 15mL Ni-NTA resin column equilibrated with lysis buffer. The column was then washed with 5 column volume of wash buffer (50mM tris-HCl, 300mM NaCl, 10mM IMD, 5% glycerol, pH 7.5). The brownish protein was eluted by gradient elution with 5 column volume of wash buffer and 5 column volume elution buffer (50mM tris-HCl, 300mM NaCl, 200mM IMD, 5% glycerol, pH 7.5). SDS-PAGE was applied to analyze the desired clean protein fractions, which were then combined an d set for overnight dialysis with dialysis buffer (50mM tris-HCl, pH 7.5, 5% glycerol) with slow stirring. The pooled fractions were concentrated using an Amicon centrifugal filter with a 30kDa molecular weight cut off (MWCO). The collected protein was further purified with 20mL packed Q-sepharose column equilibrated with lysis buffer (50mM tris-HCl, 5% glycerol, pH 7.5). The column was then washed with 5 column volume of wash buffer (50mM tris-HCl, 200mM NaCl, 5% glycerol, pH 7.5). The brownish protein was eluted by gradient elution with 5 column volume of wash buffer and 5 column volume elution buffer (50mM tris-HCl, 700mM NaCl, 5% glycerol, pH 7.5). SDS-PAGE was applied to analyze the desired clean protein fractions, which were then combined for reconstitution (Figure 4). Figure 4. SDS-PAGE gel electrophoresis analysis of MJ0841 purification fractions Reconstitution of the [4Fe-4S] Cluster of MJ0841 in Vitro The above apo-protein (16uM, 40ml) was incubated with final concentration of 5mM DTT for 1h with slow stirring at room temperature. Then, cystein was added into the above solution by dropwise to reach 10 molar folds excess of protein. After 30mins incubation, 10 molar excess of Fe(NH4)2(SO4)2 was added slowly to provide enough iron content for iron sulfur cluster and incubated for 30mins. The resulting solution was incubated with 10 molar excess of Na2S finally, and the brownish protein solution changed to dark brown after adding Na2S. The above final protein solution was kept in 4oC overnight around 14 hours for building up enough [4Fe-4S] clusters. In order to remove the unbounded iron and sulfur, the overnight reconstituted protein was concentrated to 2.5 – 3ml of final volume and loaded onto a 5mL PT10 column equilibrated with lysis buffer (50mM tris-HCl, 5% glycerol, pH 7.5), and the final pure protein was combined. Reduction of [4Fe-4S] Cluster UV-vis spectroscopy was applied here for detecting the reduction of [4Fe-4S] cluster. A characteristic peak for [4Fe-4S] cluster was shown up near 412nm before reducing. Sodium Dithionite was used as the reducing agent and the stock solution was prepared freshly right before adding into the protein. 100 equivalents of sodium dithionite were mixed with concentrated protein, and the peak at 412nm was reduced (Figure 5). According to the results from UV-vis, the 4Fe-4S cluster was built up by reconstitution, also the [4Fe-4S]2+ was reduced to [4Fe-4S]+ by the reduction of sodium dithionite. Figure 5. UV-vis spectra of purified reconsituted MJ0841 Figure 6. EPR spectrum of as-isolated MJ0841 (blue trace) and purified reconstituted MJ0841 originating from a [3Fe-4S]+ cluster. reduced with 100 equiv sodium dithionite (red trace) EPR samples preparation and spectral collection EPR spectrums of as-isolated, oxidized and reduced form of MJ0841 are detected at 10K. All samples are prepared anaerobically. As-isolated protein was prepared with the protein without reconstitution, and exhibits a strong isotropic EPR signal, which is centered at g=2.01 same as the g=2.01 signal of the [3Fe-4S]+ cluster form (Figure 6). The oxidized form protein containing [4Fe-4S]2+ was prepared by injecting 200ul concentrated protein purified through PD10 column into EPR tube, and it normally shows silent EPR signal (Figure 7). Reduced form of as-isolated protein was performed by mixing with 100 equivalents of sodium dithionite with 200ul concentrated protein, which gives the reduction form of [4Fe-4S]+ cluster and shows the characteristic EPR signal with g factors of g=2.03 and g=1.92 (Figure 8). Figure 7. EPR spectrum of oxidized form of MJ0841. Figure 8. EPR spectrum of reduced form of MJ0841. SAM cleavage activity of MJ0841 The characteristic reaction for detecting radical SAM enzymes is reductive cleavage of SAM into S-adenosylhomocysteine (SAH) and 5’-deoxyadenosyl radical (5’dAdo). Assays were conducted under strict anaerobic conditions. The reaction assay contains the following: 50mM Tris-HCl, 5mM DTT, 5Mm sodium dithionite, 0.5mM SAM. Reactions were initiated by addition of SAM and carried out at 20oC for 20 hours. The control reaction was run under the same conditions as the above assay, but without presence of protein. Trifluoroacetic acid (TFA) (final concentration 5% v/v) was added to quench the reactions, which then were identified by HPLC analysis. HPLC analysis of SAM cleavage assay products After quenching by TFA, the reaction mixture was centrifuged and the supernatant was applied into HPLC analysis. 10ul of assay mixture was injected into C18 column, which had been pre-equilibrated with equilibrium buffer (40mM ammonium acetate, pH=6.2). Then the column was washed with a linear gradient from 0-50% acetonitrile for 30mins at room temperature to detect SAH and 5’dAdo. The UV-detector was set at wavelength 258nm, and the standard samples, SAM, SAH, and 5’dAdo, were run with the same condition as SAM cleavage reaction products. According to the retention time comparison between standard samples and products, formation of SAH and 5’dAdo were all detected via HPLC analysis when enzyme was present. In the absence of enzyme MJ0841, SAM was not consumed at all and there were no any products peak formed, which confirmed the SAM was cleaved by enzyme. The dark blue, red, light blue traces show the relative intensities of 5’dAdo, SAH, and SAM standards. The green trace shows the assay with the use of reconstituted MJ0841, and the SAH and 5’dAdo were both observed. The purple trace shows the control assay without MJ0841, and there was not any of 5’dAdo formed (Figure 9). Figure 9. HPLC analysis of the SAM cleavage assays Conclusions Future Work Initial results seem to show the [4Fe-4S] cluster and the enzyme activity. Since SAM was cleaved enzymatically, the products will be detected by mass spectroscopy to confirm the formation SAH and 5’dAdo. Furthermore, probable substrates of MJ0841 will be prepared, which should contain the amino acids that would be modified. The activity assays with substrates will be examined to detect the desired methylation reaction on substrates.

Sunday, January 19, 2020

Compare contrast essay Essay

Novelist Paul Sheldon has plans to make the difficult transition from writing historical romances featuring heroine Misery Chastain to publishing literary fiction. Annie Wilkes, Sheldon’s number one fan, rescues the author from the scene of a car accident. The former nurse takes care of him in her remote house, but becomes irate when she discovers that the author has killed Misery off in his latest book. Annie keeps Sheldon prisoner while forcing him to write a book that brings Misery back to life. Compare & Contrast Essay â€Å"A Rose for Emily† by: William Faulkner & â€Å"Misery† by: Stephen King I’ve chosen to compare & contrast â€Å"A Rose for Emily† By: William Faulkner with â€Å"Misery† By: Stephen King, Obsessive women, broken ankles, trapped men & Suspenseful endings both of these stories have a lot in common. In Stephen King’s â€Å"Misery† A Famous Novelist named Paul Sheldon is transitioning his writing from Romance to Fiction. While on his way to Colorado from his winter hide away he is in a horrific car accident brought on by the harsh weather conditions. Thankfully the writer’s number one fan and former nurse Annie Wilkes lived nearby and saved Paul from freezing to  death. Annie Wilkes just like Miss Emily Grierson lived isolated and away from the world, though Miss Emily lived in the town & Annie Wilkes didn’t Miss Emily Isolated herself socially by not leaving her home nor opening the door when people of the town came by. Annie However, actually lived in an isolated cabin in the mountains. In â€Å"A Rose for Emily† people of the town feel sorry for her because after losing her father (page: 207 she was sick for a long time), But not long after they began seeing her with a gentle man named Homer Barran. A construction worker who had come into town to pave the sidewalks. After a year of being with Homer the towns folk began saying they would marry even though Homer claimed to like men (page: 209, He was not a marrying man). One evening after  ending their relationship Homer went to see Miss Emily using rat poison she killed him and let everyone believe he left and went on with his life while she hid his remains. Annie Wilkes didn’t kill Paul Sheldon, but took advantage of the fact that he had been put in critical condition due to the car accident she removed him from. For weeks she held him captive until he gave her favorite novel the proper ending it deserved. After noticing his several, sneaky attempts to escape Annie straps Paul to the bed and cripple s him by breaking both his ankles with a sledge hammer. Both of these women felt the need to keep these men hidden, trapped, and injured so they may stay with them forever. Miss Emily trapped Homer in death to stay with her since he had no interest in marrying her. In â€Å"Misery† Prior to having his ankles broken Paul finds newspaper clippings of previous victims of Annie where she was never convicted, He then realizes he may be next on her list. Soon after crippling Paul an officer drops by to see if Annie was aware that Novelist Paul Sheldon was missing and they were searching for him. Paul hears the officer and yells for help when Annie hears his screams she kills the officer and Paul is left once more to endure her gruesome punishment. Neither one of the men could predict what was going to happen to them, but Homer didn’t endure being tortured nor was he held captive for weeks. In â€Å"A Rose for Emily† Miss Emily was never caught nor suspected for the  disappearance of Homer. After the death of Miss Emily and after her services the town folk were curious to see   the inside of her home. (page 211, there was one room that no one had seen in forty years, and which would have to be forced). After entering the room they noticed mens toiletries, suits, ties and a pair of shoes even more so they noticed the man in her bed, His well over rotten body lay there in a nightshirt and next to him they could see where Miss Emily would lay beside him every night after his death. Unlike Miss Emily Annie didn’t get away with what she did to Paul, after killing the officer Paul tricks her into buying Champagne and cigars to celebrate the return of Misery, while setting up for their night of celebration Paul lights the re-written novel on fire and throws it. Annie begins to put out the flame when Paul slams his typewriter on the top of her head and shoves pieces of the burned novel pages into her mouth, she then dies of a fractured skull. Sometime after Paul is rescued by police. Both of these stories where great and though they are in different settings the characters shared similar physiological problems and depression. I think if both of these women met and could ever be in the same story there would be gory, suspenseful, torturing of men. I would like to read a book where Stephen King and William Faulkner write a story sharing both their ideas, I think it would be very interesting, thrilling and great to read.

Saturday, January 11, 2020

Dota Thesis Essay

Here in the Philippines, we believe in the saying of our national hero Dr. Jose P. Rizal that â€Å"Youth is the hope of our Mother land†. In their hand lies the future generation to follow. But as we see from the situation nowadays, what we are expecting from the youth is the contrary if the saying. Teenagers are prone to pregnancy. Almost every year there is a rapid increase on the number of pregnant youths. One of the major causes of this problem is that most of the youths nowadays grew up in broken homes. Parents either the father or mother of the youths chose to leave their children behind because of the fact that they cannot handle anymore their obligation as parents. In relation to this problem, our government is now working on the Reproductive Health Bill (RH Law) as one of the most effective solution not only to teenage pregnancy but also to the increasing population of our country. The literature review was conducted to identify risk factors associated with teenage pregnancy and parenthood, including patterns of teenage sexual behaviour, as well as current knowledge about effective strategies in teenage pregnancy prevention. The impact of teenage parenthood on parents and children and ways of supporting pregnant and parenting young women was also explored. The review relied predominantly on Australian literature in order to reflect local issues and perspectives. However, the need to consider more recent or extensive research in some areas required the use of overseas literature, mainly from the US and the UK.Teenage pregnancies in the Philippines surged by 70 percent over one decade, a ranking official of the United Nation Population Fund Agency (UNFPA). Ugochi Daniels, UNFPA country representative, said the rising number of teenage pregnancies in the country is an area of concern that the agency is giving serious attention to, exposing as it does adolescent girls (10 to 19 years old) to high risk. Government statistics on female adolescent pregnancies indicate that the total number of annual births changed little over the preceding 10 years, but the number of teenage pregnancies rose 70 percent, from 114,205 in 1999 to 195,662 in 2009. Based on data compiled from birth certificates, of the 1.75 million live births in 2009, the latest review year, over 11 percent involved teenage mothers. Teenage pregnancy is widespread in the Philippines, especially amongst the poor. It is astory that may not be too pleasant, but one that is repeated all too often.Teen pregnancy is anobvious rising issue in our society. During the 21 st century, the statistics about this issue continues to rise even though there are a lot of disadvantages that young mothers and fathers can obviously get in the said issue. Fox (2008) defined teenage pregnancy as: Teenage pregnancy is one of the most important and alarming issues in today’s world. A teenage pregnancy, also referred to as teen pregnancy, comes to be when a girl attains motherhood before she becomes a major or adult, as laid down by the law of the land Some 16.5 million Filipinos belong to the 15-24 year old age group. We are forced with a glaring truth that at a very young age, a lot of young people today have children of their own. 30% of all births belong to this age group; and by the age of 20, 25% of the youth are already mot hers. This shocking truth about teenage pregnancy in the Philippines should wake the government and parents themselves to take action (Flores, 2008). According to the 2002 Young Adult Fertility and Sexuality Study by the University of the Philippines Population Institute (Uppi) and the Demographic Research and Development Foundation, twenty six(26%) of our Filipino youth nationwide from ages 15 to 25 admitted to having a premarital sex experience. What ¶s worse is that 38 percent of our youth are already in alive-in arrangement. Another is the 1998 National Demographic and Health Survey (NDHS)reveals that 3.6 million of our teenagers (that’s a whopping 5.2 percent of our population!) got pregnant. In 92 percent of these teens, the pregnancy was unplanned, and the majority, 78 percent, did not even use contraceptives the first time they had sex(Singsong, 2008).Most teenagers don’t plan to get pregnant, but many do. Teen pregnancies carry extra health risks to the mother and the baby. Risks for the baby include premature birth and a low birth weight. To control this, we must help each other by following the suggested solutions. I. Cause Causes of teenage pregnancy are occurring due to following reasons according to Fox (2008): Consequence of raging hormones. Most teenagers experience sudden and hitherto unknown emotions and feelings during their early puberty. All this, coupled with a sudden sense of new-found freedom and sexuality, results in many of them giving vent to their feelings through sexual expressions and experiences. Of course, most countries stress on sex education in schools, but yet, some teenagers involve themselves in unsafe sex, which could lead to unwanted pregnancies. Peer pressure. A lot of teenagers indulge in early sexual behaviour due to peer pressure. This is due to the fact that they feel the great need to be ‘hip’ and ‘accepted’ by their circle of friends. The only way they could probably achieve that would be by having a boyfriend or girlfriend or at least by dating and indulging in sexual acts often. Contraceptive Failure. Though there are many types and brands of contraceptives and emergency birth control pills easily available in the market, these are never a guarantee for complete safety. Only Mother Nature knows when she might want to get into action, so no form of birth control is going to be completely foolproof! (Fox, July 18, 2008).Other causes of teenage pregnancy are: 1. Lack of Parental Guidance. Most people evade their children from talking about sex. In some cases, they provide false information regarding sex and discourage their children to participate in any informative discussion about sex. In some cases, teenage mothers are not well educated about sex before getting pregnant and thus this leads to lack of communication between the parents and the children. 2. Inadequate Knowledge about Safe Sex. Most adolescents are unaware of safe sex. They probably have no access to the traditional methods of preventing pregnancy. And the main reason behind is that they are either too embarrassed or fear to seek information about it. It was found that more than 80% of teenage pregnancies are unintended. 3. Exploitation by Older Men. This is another major factor that contributes to pregnancyamong the teenagers. Those girls who date older men are more likely to become pregnant before they attain womanhood. Rape, sexual exploitations etc. also takes place th at leadsto unwanted pregnancy among teenage girls. 4. Socio Economic Factors. Teenage girls who belong to the poor families are more likelyto become pregnant. Researchers have found that even in the developed countries teenage pregnancy occurs most commonly among the deprived sections. 5. Glamorization of Pregnancy. The movie industry and the media contribute to teenage pregnancy by glamorizing teen pregnancy in news stories and movies. Movies that depict teen pregnancy as something to be desired encourage teens to engage in reckless sexual activity, according to ABC’s â€Å"Good Morning America.† During adolescence, teens become more focused on their appearance and how their peers perceive them. They want to be seen as part of the group, so if teen pregnancy is viewed as acceptable in their school or amongst their friends, they may seek to become pregnant as a way to gain social acceptance. 6. Teenage Drinking. Teen drinking can cause an unexpected pregnancy, according to the website Love to Know. Many teens experiment with drugs and alcohol. Drinking lowers a teen’s ability to control her impulses, contributing to 75 percent of pregnancies that occur between the ages 14 and 21. Approximately 91 percent of pregnant teens reported that although they were drinking at the time, they did not originally plan to have sex when they conceived. 7. Sexual Abuse or Rape. Teens can become pregnant as a result of sexual abuse or rape. The Guttmacher Institute states that between 43 and 62 percent of teens acknowledge that they were impregnated by an adult male, and two-thirds report that their babies’ fathers are as old as 27. Approximately 5 percent of all teen births are the result of a rape. 8. Inconsistent use of birth control Even though the use of contraceptives has increased among teenagers, its use remains spotty. Teens may use birth control to help prevent teen pregnancy, but most do not use contraceptives consistently. Erratic contraceptive use, however, is better than none. A sexually active teen that does not use any birth control has a 90 percent chance of becoming pregnant within a year. Some of the reasons that birth control is not used include the following: uneducated about birth control, uncomfortable using birth control methods and unable to access reliable birth control. 9. Abstinence-only sex education. Despite recent government efforts to fund abstinence-only sex education, the vast majority of teenage boys and girls (62 percent and 70 percent) have initiated vaginal sex by the age of 18. Because of their nature, abstinence-only programs leave out educational information on birth control. These programs have never been shown to actually reduce the incidence of teen pregnancy. However, they are the programs that receive the most funding from the government. There are state governments that forbid any sort of teen sex education in schools at all. This means that teens that experiment may not have information on birth control and are more likely to engage in activity that results in teen pregnancy. 10. Psychological factors. The immature and irresponsible behaviour arising duet complex teenage psychology is another important cause of teenage pregnancies. Teenagers often go through a number of emotions because of their own transition from childhood and peer pressure. The causes above are also similar to the causes here in the Philippines. II. Solution a) Existing Solutions – since abortion is immoral and legally wrong here in the Philippines, they consider adoption for the baby or acceptance for the parents. If not yet born, then they suggest that the baby not be killed. Currently, the government proposes sex education but it is still debated whether it would be taught or not. One way to prevent pregnancy is by practicing safe sex. In addition to it, birth control tools such as condoms, pills, intra-uterine devices or supremacies can regulate the fertility of a woman’s eggs. Even though it decreases the chances of not being pregnant, still it is not 100%sure and effective to avoid pregnancy. Some are even desperate that they will come to a point that they have to abort their babies without knowing the effects it may cause to the mother and it will kill the conscience of who will ever do this abortion thing. With the help of Science, Medicine has developed a new pill contraceptive namedRU486, the morning after solution to a sex encounter. Teenagers nowadays are sexually active without their parents knowing their sexual activities. Often times, media promotes sexuality to the young which may cause them to be mislead with wrong information about sex and they might be misguided, that’s why parents play an important role to the lives of their children. For all we know, sex is for those couples who already undergo to the Sacrament of Marriage, because doing sex needs a lot of sense of maturity not only physical but mental and spiritual basis. Once a pregnancy occurs from premarital sex, the couple is bound to live together for the rest of their lives with or without marriage because they have now the obligation to raise the child. The best way to avoid teen pregnancy is by avoiding or abstaining from sex before marriage. One thing is for sure, abstain from sex will keep you 100% safe from diseases, undesired relationship, and of course teenage PREGNANCY (Life Challenges -AllAboutLifeChallenges.org, 2011). Other possible Solutions are: 1. Abstinence from sex until you are old enough to do so. 2. Usage of contraceptives and other forms of birth controls. 3. Sex Education. 4. Affection and Guidance of parents. 5. Counselling. 6. Discouraging early dating What Ever the solutions may be, we must all keep in mind that we must be pro-life. b) How it is done in other countries. Existing solutions do not only exist on the Philippines but also on other parts of the world. For Example, abortion in the United States has been legal in every state since the United States Supreme Court decision in Roe V. Wade, on January 22, 1973. Prior to â€Å"Roe†, there were exceptions to the abortion ban in at least 10 states; â€Å"Roe† established that a woman has a right to self-determination (often referred to as a â€Å"right to privacy†) covering the decision whether or not to carry a pregnancy to term, but that this right must be balanced against a state’s interest in preserving fetal life (Wikipedia, 2011). In Egypt, they teach knowledge about male and female reproductive systems, sexual organs, contraception and STDs in public schools at the second and third years of the middle-preparatory phrase (when students are aged 12-14)(Wikipedia, 2011). In France, They promote sex education and it has been part of school curricular since 1973. Schools are expected to provide 30 to 40 hours of sex education, and pass out condoms, to students in grades eight and nine (Wikipedia, 2011). In Britain, which has one of the highest teenage pregnancy rates in Europe ,sex education is a heated issue in government and media reports In Sweden, sex education has been a mandatory part of school education since 1956. The subject is usually started between ages 7 and 10, and continues up through the grades, incorporated into different subjects such as biology and history In some countries, such as America, Falcon punch is done by the teens. The Falcon Punch is most often used to end the untimely pregnancy of a loved one in a humane manner, especially if the loved one happens to be under the age of sixteen. (EncyclopediaDramatica,2011). Active euthanasia, commonly referred simply as euthanasia, is only legal in the Netherlands and Belgium. (Wiki Answers, 2011) Euthanasia is the intentional killing by act or omission of a dependent human being for his or her alleged benefit, and over the years it has been one of the favourite tools of those who think society is better off when we take active steps to kill the poor, the disabled, the elderly, or the  ³unwanted ´. Today the debate over euthanasia most commonly revolves around the issue of physician-assisted suicide. There are many forms of euthanasia but the assisted suicide is its most common from. This method has also been done to prevent teenage pregnancy. (Wikipedia,2011) There are many possible solutions that can be done, some countries perform different methods that are immoral for Filipinos. One example is abortion. But amidst all this, we must learn to give importance to life because it is God’s wonderful gift to all of us STATEMENT OF THE PROBLEM: This study answers the following questions: 1. What is your reaction on teen pregnancy? 2. Do you know anything about RH Law? 3. Did you ever think of aborting a baby? 4. Do you still continue to pursue your education? 5. Do you know of anywhere in your local area where free condoms are available to younger couples? 6. What is the best solution or prevention on Early Pregnancy? 7. How the other country preventing early pregnancy. Locale of the Study The Study was done in STI College Laguna, The IT students give an survey about their reaction about Early Pregnancy and what they say about passing the RH Law. Importance: The fact is that the RH Bill is now a law of the Republic, and the President, barring judicial intervention, has the obligation of seeing to its effective, just and balanced implementation. Indeed, it would be tragic if, after all the acrimony, we end up with a law that is not implemented like many good legislation in this country. According to Dean Tony La Vina, in her view, the protracted debate, even if it was too long (lasting 13 years), actually improved the bill and, thanks in part to the opponents of the bill, we have a much more balanced and a better law than originally proposed. Among others, it is clear that abortifacient methods are prohibited (although the definition of abortion as preventing implantation of a fertilized ovum is controversial), freedom of conscience is respected, and there is neither a mandate to reduce our population nor a preference for smaller families. Below is a summary of what I believe are the most important provisions under the RH Law: * It affirms in a balanced way various state policies related to the right of health, including reproductive health, the rights of women, couples and families, and the protection of the life of the mother and the unborn. The law provides for spousal and parental consent in specific instances although safeguards to protect the individual rights of mothers or those availing of a service or procedure are also included. * In my view, the RH Law’s most important provision is the guarantee by the State to provide â€Å"universal access to medically-safe, non-abortifacient, effective, legal, affordable, and quality reproductive health care services, methods, devices, supplies which do not prevent the implantation of a fertilized ovum as determined by the Food and Drug Administration (FDA) and relevant information and education thereon according to the priority needs of women, children and other underprivileged sectors.† * In implementing the above policy, the State shall promote and provide information and access, without bias, to all methods of family planning, including effective natural and modern methods which have been proven medically safe, legal, non-abortifacient, and effective in acco rdance with scientific and evidence-based medical research standards. If this â€Å"no bias† principle is actually implemented, it will go a long way in reducing acrimony in the law’s implementation. * The RH law also provides that the State shall also provide funding support to promote modern natural methods of family planning, especially the Billings Ovulation Method, consistent with the needs of acceptors and their religious convictions. * The RH Law does not set demographic or population targets, and in fact, states that the mitigation, promotion and/or stabilization of the population growth rate is incidental to the advancement of reproductive health. Further, each family has the right to determine its ideal family size: although the State is mandated to equip each parent with the necessary information on all aspects of family life, including reproductive health and responsible parenthood, in order to make that determination. * Religious freedom is actually respected in the RH Law. Hospitals owned and operated by a religious group do not have to provide services contrary to its beliefs although these hospitals shall immediately refer the person seeking such care and services to another health facility, which is conveniently accessible. The conscientious objection of a health care service provider based on his/her ethical or religious beliefs are also respected, accompanied by an obligation for referral. These exceptions do not, however, apply in emergency cases. * The RH Law provides that no person shall be denied information and access to family planning services, whether natural or artificial. Minors however will not be allowed access to modern methods of family planning without written consent from their parents or guardian/s except when the minor is already a parent or has had a miscarriage. * Finally, age- and development-appropriate reproductive health education to adolescents (10-19 years) is mandated for public schools, which program shall be based on consultations with parents-teachers-community associations, school officials and other interest groups. Private schools may adopt the program at their own option, but consistent with religious freedom, are not obliged to do so. Scope and Limitations: This investigation is conducted to determine the rate of STI students that is reacted on Early or Teen Pregnancy. This way, we could determine the status and profile of the teenager Reaction in teenage pregnancy. The aspects looked into were the quantitative information of number of STI IT students Reaction in teenage pregnancy and the data behind this. This investigation focuses on number of IT students Reacted in teenage pregnancy in STI College Laguna portrayed by our group as respondents during February 2013. Definitions of Terms Teenage pregnancy – refers to any pregnancy on women that took place between the ages 13 – 19 years old. Teen aged Students – refers to students that ages a number that end in â€Å"teen† as the last syllable such as13, 14, 15, 16, 17, 18, and 19. Rate – refers to a quantity from a recent quantity STIS – refers to Sexually Transmitted Infections STDS – refers to Transmitted Diseases Sex – the sum of the structural, functional, and behavioural characteristics of organisms that are involved in reproduction marked by the union of gametes and that distinguish males and females RH – Reproductive Health Contraceptives – A device or drug serving to prevent pregnancy. Reference: http://www.dcsi.sa.gov.au/pub/ http://www.scribd.com/doc/51595306/Teenage-Pregnancies-in-the-Philippines-2, http://www.rappler.com/thought-leaders/18972-reconciliation-post-rh-law